AB72110-EN-WINE-Compendium-0816M.pdf

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AB72110-EN 0816M-W5
Overview:
Red wine is a very complex mixture and a rich source of beneficial anti-oxidants.
Identification and quantitation of these natural products is challenging. Ultra High
Pressure Liquid Chromatography (UHPLC) coupled to a mass spectrometer enables
simultaneous detection and relative quantitation of wine’s antioxidant constituents and
progressive changes after exposure to air. This application note demonstrates the
feasibility of analyzing complex mixtures without any prior sample preparation by
making use of the high resolving power of both UHPLC and the Orbitrap mass
analyzer detector.
Method:
Chromatography of wine samples was performed using a Thermo Scientific™ Accela™
UHPLC with a Thermo Scientific™ Hypersil GOLD™ 1.9µm 100 x 2.1mm column.
Detection was carried out using a Thermo Scientific™ LTQ Orbitrap XL™ mass
spectrometer, with Thermo Scientific™ SIEVE™ 1.2 software.
Part Number Description
25002-102130 Hypersil GOLD 1.9µm 100 x 2.1mm
Conclusion:
UHPLC affords fast analysis times while maintaining very high chromatographic
resolution (peak width 7 seconds at half height). Accurate mass measurements
significantly improves the precision of quantitation by eliminating nearly all isobaric
interferences. This is a particularly important aspect for complex mixture analyses such
as red wine.
Direct Analysis of Red Wine Using Ultra-Fast
Chromatography and High Resolution Mass
Spectrometry
Eugen Damoc, Michaela Scigelova, Anastassios E. Giannakopulos, Thomas Moehring,
Thermo Fisher Scientific, Bremen, Germany
Frantisek Pehal, NNH Hospital Na Homolce, Prague, Czech Republic
Martin Hornshaw, Thermo Fisher Scientific, Hemel Hempstead, UK
Application Summary AN 30173