AB72110-EN-WINE-Compendium-0816M.pdf

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AB72110-EN 0816M-W2
Overview:
The analysis of pesticide residues in red wine is challenging due to the complexity of
the matrix, which contains alcohol, organic acids, sugars, and polyphenols.
Sample preparation involves the extraction of pesticides from red wine using the
QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) extraction method.
The samples then undergo cleanup by dispersive solid-phase extraction (dSPE) using
primary secondary amine (PSA) sorbent, which effectively retains organic acids,
sugars, and phenolic pigments.
Method:
A modified QuEChERS (AOAC version), extraction method was used along with
dispersive solid-phase extraction (dSPE). The purified extract was separated using the
Thermo Scientific™ Accucore™ aQ 2.6µm 100 x 2.1mm HPLC column and Thermo
Scientific ™ Accucore ™ aQ Defender™ 2.6µm 10 x 2.1mm guard column to facilitate fast
and high efficiency separations on a Thermo Scientific™ Dionex™ UltiMate ™ 3000 LC
system. Detection was carried out on a Thermo Scientific™ TSQ Vantage™ tandem
mass spectrometer in ESI+ mode. Data processing was carried out using Thermo
Scientific ™ TraceFinder™ software.
Part Number Description
60105-335 Mylar Pouch with 6g MgSO4 and 1.5g Sodium Acetate
60105-350 2mL Centrifuge Tube with 150mg MgSO4 and 150mg PSA
17326-102130 Accucore aQ 2.6µm 100 x 2.1mm
17326-012105 Accucore aQ Defender Guard 10 x 2.1mm
Conclusion:
A fast, easy and cost-effective method has been successfully developed using the
QuEChERS-based approach. The Accucore aQ HPLC columns gave good resolution
and peak shapes for all of the pesticides. Good linearity, low LOQs, and satisfactory
accuracy and precision data were obtained, indicating that this method is suitable for
pesticide residue analysis in red wine.
Determination of 24 Pesticide Residues in Red
Wine Using a QuEChERS Sample Preparation
Approach and LC-MS/MS Detection
Mike Oliver, Thermo Fisher Scientific, Runcorn, UK
Application Summary AN 20830