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AB72110-EN 0816M-W9
Overview:
Sulfur dioxide (SO
2
) is added to control the process of wine making. The presence of
total SO
2
, both free and bound, is regulated and, as a result, a warning statement is
required on wine labels because sulfite, is considered an allergen. The European Union
established a maximum permitted level of total SO
2
in wine of 500 mg/L, in the USA,
the maximum level of total SO
2
permitted is 350 mg/L.
An automated method to measure free SO
2
in wine samples is presented.
Method:
The method is based on the reaction between sulfur dioxide,
p
-rosaniline
hydrochloride, and formaldehyde. This method is designed to use optimal reagent
concentrations and volumes to provide accurate results.
A Thermo Scientific
™
Gallery
™
discrete photometric analyzer was used for this study.
The SO
2
Free system reagents kit for Gallery analyzers were used.
Conclusion:
The automated photometric Thermo Scientific SO
2
Free method correlates well with
the FIA method. This new automated SO
2
Free method is very quick and easy to use.
Analysis of 60 samples takes only 35 min and allows simultaneous analysis of various
sugars and acids, color, and total SO
2
. Compared to the FIA method, the photometric
method requires only small volumes of reagents, thus being the more economically
and environmentally friendly choice. This method enables a laboratory to fully automate
SO
2
determinations and replace traditional time consuming reference and distillation
methods.
Read the full application noteFast and Accurate Automated Method for
Free Sulfite Analysis in Wine
Eric Naigeon,
1
Marco Rastetter,
2
Mari Klemm,
3
and Annu Suoniemi-Kähärä3
1
Thermo Fisher Scientific, Paris, France,
2
Thermo Fisher Scientific, Dreieich, Germany
3
Thermo Fisher Scientific, Vantaa, Finland
Application Summary AN 71451