AI-64310-CRFT

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AN63880_E 12/13S
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Standardization (Organisation Internationale De Normalization). RECIPE and ClinMass are registered trademarks of RECIPE Chemicals +
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Application Note 593
Conclusion
The analysis of benzodiazepines for research purposes
presents a challenge due to their low concentrations, their
short half life, and their diversity. In this work, the
RECIPE ClinMass® complete kit for the quantification
of 33 benzodiazepines and metabolites in serum was
evaluated for research use with a TSQ Vantage triple
quadrupole. The online SPE configuration was achieved
by using a Transcend TLX-1 system. HPLC coupled to
tandem mass spectrometry may be a suitable tool for the
quantification of benzodiazepines in research applications
since it can deal with the differences in polarities of the
benzodiazepines and their metabolites. The use of SRM
detection increases the analytical specificity needed to
attain the low quantification ranges for these types of
molecules. Finally, the use of LC-MS/MS simplifies sample
preparation since the matrix clean-up is performed by
online SPE.
This research method showed good results in terms of
variability, precision, and dynamic range requirements for
a clinical research method.
For Research Use Only.
Not for use in diagnostic procedures.
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