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3

Table 2. SRM parameters used for the analysis

Compound

Retention Time

(minutes)

Precursor

Ion

Product

Ion

S-Lens

Collision

Energy

7-Aminoclonazepam

4.56

286.1

222.2

114

24

7-Aminoflunitrazepam

4.70

284.1

135.1

101

26

7-Aminonitrazepam

4.56

252.1

121.1

96

26

α

-OH-Alprazolam

6.20

325.1

297.1

102

24

α

-OH-Midazolam

7.24

342.1

324.1

102

19

α

-OH-Triazolam

5.95

359.1

331.0

101

23

Alprazolam

6.66

309.1

205.1

112

38

Bromazepam

5.73

316.0

182.1

100

31

Chlordiazepoxide

7.64

300.1

227.1

95

24

Clobazam

6.35

301.1

259.1

96

19

Clonazepam

6.10

316.1

270.1

114

23

Demoxepam

5.67

287.1

178.0

91

21

Desalkylflurazepam

7.02

289.1

140.1

123

28

Desmethylflunitrazepam

5.80

300.1

254.1

95

23

Diazepam

8.47

285.1

193.1

91

28

Estazolam

6.31

295.1

267.1

119

22

Flunitrazepam

6.20

314.1

268.1

89

25

Flurazepam

9.16

388.2

315.1

117

20

Lorazepam

6.67

323.0

277.1

100

21

Lormetazepam

7.32

335.1

289.1

88

21

Medazepam

10.57

271.1

207.1

86

26

Midazolam

8.41

326.1

291.1

129

25

Nitrazepam

6.20

282.1

236.1

95

23

Norclobazam

5.96

287.1

245.1

81

19

Nordiazepam

7.98

271.1

140.1

99

27

Oxazepam

6.76

287.1

241.1

106

21

Prazepam

10.03

325.1

271.1

100

21

Temazepam

7.12

301.1

255.1

96

22

Tetrazepam

9.96

289.1

225.2

117

28

Trazodone

9.34

372.2

148.1

115

32

Triazolam

6.49

343.1

308.1

146

25

Zaleplone

5.43

306.1

236.1

100

26

Zolpidem

7.10

308.2

235.2

116

33