230 / 658
4
Table 3. Calibration parameters and LOQ
Compound
Internal Standard
Weighting
R²
LOQ (µg/L)
7-Aminoclonazepam
7-Aminoclonazepam-D4
1/X
0.999
2.6
7-Aminoflunitrazepam
7-Aminoflunitrazepam-D7
1/X
2
0.995
2.6
7-Aminonitrazepam
7-Aminoclonazepam-D4
1/X
0.995
22.1
α
-OH-Alprazolam
α
-OH-Alprazolam-D5
1/X
0.993
2.7
α
-OH-Midazolam
α
-OH-Midazolam-D4
1/X
0.995
10.8
α
-OH-Triazolam
α
-OH-Triazolam-D4
1/X
0.997
4.6
Alprazolam
Alprazolam-D5
1/X
0.996
1.1
Bromazepam
7-Aminoflunitrazepam-D7
1/X
0.997
15.8
Chlordiazepoxide
Chlordiazepoxide-D5
1/X
2
0.993
111.5
Clobazam
Triazolam-D4
1/X
2
0.992
9.5
Clonazepam
Clonazepam-D4
1/X
2
0.994
2.5
Demoxepam
Clonazepam-D4
1/X
0.997
216.0
Desalkylflurazepam
Temazepam-D5
1/X
2
0.993
9.7
Desmethylflunitrazepam
Clonazepam-D4
1/X
2
0.990
4.5
Diazepam
Diazepam-D5
1/X
2
0.993
20.4
Estazolam
Estazolam-D5
1/X
0.996
21.4
Flunitrazepam
Flunitrazepam-D7
1/X
0.990
5.2
Flurazepam
Prazepam-D5
Equal
0.996
4.3
Lorazepam
Lorazepam-D4
1/X
2
0.996
20.6
Lormetazepam
Nordiazepam-D5
1/X
2
0.989
1.8
Medazepam
Nordiazepam-D5
Equal
0.997
8.6
Midazolam
Midazolam-D4
Equal
0.995
15.5
Nitrazepam
Nitrazepam-D5
1/X
2
0.997
10.5
Norclobazam
α
-OH-Triazolam-D4
1/X
0.997
133.0
Nordiazepam
Nordiazepam-D5
1/X
0.997
16.7
Oxazepam
Oxazepam-D5
1/X
0.995
62.5
Prazepam
Prazepam-D5
1/X
0.999
41.7
Temazepam
Temazepam-D5
1/X
2
0.996
21.7
Tetrazepam
Prazepam-D5
Equal
0.999
8.2
Trazodone
Prazepam-D5
1/X
0.997
82.5
Triazolam
Triazolam-D4
1/X
0.988
4.0
Zaleplone
7-Aminoflunitrazepam-D7
1/X
0.999
4.2
Zolpidem
Zolpidem-D6
Equal
0.998
21.2