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Results and Discussion
Opiates Analysis
The limits of quantitation were 10 ng/mL and calibration
ranges were 10–6000 ng/mL for all opiates. Figure 6
shows representative calibration curves for selected
opiates. Figure 7 shows representative chromatograms at
10 ng/mL for all opiates tested. Intra- and inter-assay
quality control statistics shown in Table 3 demonstrate the
method to be reproducible across the calibration range for
the opiates. Limited matrix effects were seen, and those
were largely mediated by deuterated internal standards
(Table 4). The data collected with this method correlated
well with data collected using an LC/MS method
previously validated in a collaborating laboratory (Figure 8).
Figure 6. Calibration curves for selected opiates
Figure 7. Chromatograms of the lowest opiates calibration
standard (10 ng/mL)
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