246 / 658
2
Data were acquired in selected-reaction monitoring
(SRM) mode. SRM transitions for the 43 drugs and their
internal standards are shown in Table 1. For each analyte
and internal standard, two SRM transitions were
monitored. One of transition was used as the quantifier
and the other as the qualifier. The signal ratio between the
qualifier and the quantifier was used to evaluate the
validity of the results.
Table 1. Drug analytes, their corresponding internal standards, and the SRM transitions for both analytes and internal standards
Validation
The validation procedure included tests for the following:
1) matrix effects; 2) lower limit of quantitation (LLOQ),
linear range, accuracy, and precision; and 3) carryover.
Analyte
Precursor
Ion
(
m/z
)
Quantifier
Ion
(
m/z
)
Qualifier
Ion
(
m/z
)
Ion
Ratio
(%)
Corresponding
Internal
Standard
Precursor
Ion
(
m/z
)
Quantifier
Ion
(
m/z
)
Qualifier
Ion
(
m/z
)
6-MAM
328.10
165.10
211.00
86.0
6-MAM-d3
331.20
165.10
211.10
7-Amino-clonazepam
286.00
222.10
250.10
95.0
7-Amino-clonazepam-d4
290.11
226.10
254.10
7-Amino-flunitrazepam
284.10
135.10
226.10
52.0
7-Amino-flunitrazepam-d7
291.11
138.20
230.10
7-Aminonitrazepam
252.10
121.10
224.10
16.0
7-Amino-clonazepam-d4
290.11
226.10
254.10
a
-Hydroxy-alprazolam
325.10
216.10
297.10
52.0
α
-Hydroxy-alprazolam-d5
330.10
221.10
302.10
Alprazolam
309.40
205.00
281.00
76.0
Temazepam-d5
306.10
260.10
288.10
Amphetamine
136.10
65.30
91.20
10.0
Amphetamine-d5
141.10
92.20
93.20
Benzoylecgonine
290.10
105.10
168.10
30.0
Benzoylecgonine-d3
293.10
105.10
171.10
Benzylpiperazine
177.10
65.30
91.20
16.0
Benzylpiperazine-d7
184.10
70.20
98.20
Buprenorphine
468.30
396.30
414.30
120.0
Diazepam-d5
290.12
198.10
227.10
Carisprodol
261.20
62.10
97.10
58.5
α
-Hydroxy-alprazolam-d5
330.10
221.10
302.10
Clonazepam
315.90
214.00
270.00
26.6
Temazepam-d5
306.10
260.10
288.10
Cocaine
304.10
82.20
182.10
17.0
Amphetamine-d5
141.10
92.20
93.20
Codeine
300.10
165.00
215.00
91.0
Codeine-d3
303.20
165.10
215.10
Diazepam
285.10
193.10
222.10
72.0
Diazepam-d5
290.12
198.10
227.10
EDDP
279.20
235.20
250.20
54.5
Temazepam-d5
306.10
260.10
288.10
Fentanyl
337.20
105.20
188.20
67.0
Temazepam-d5
306.10
260.10
288.10
Flunitrazepam
314.40
239.10
268.10
32.5
Temazepam-d5
306.10
260.10
288.10
Flurazepam
388.10
288.10
315.10
11.5
Temazepam-d5
306.10
260.10
288.10
Hydrocodone
300.20
171.00
199.00
34.5
MDA-d5
185.10
110.20
137.10
Hydromorphone
286.11
157.10
185.10
64.0
Benzylpiperazine-d7
184.10
70.20
98.20
Lorazepam
321.00
275.00
303.00
64.0
α
-Hydroxy-alprazolam-d5
330.10
221.10
302.10
MDA
180.10
105.20
135.10
79.0
MDA-d5
185.10
110.20
137.10
MDEA
208.10
135.10
163.00
24.0
Nordiazepam-d5
276.10
165.00
213.10
MDMA
194.10
135.10
163.10
40.0
MDMA-d5
199.10
135.10
165.10
Meperidine
248.20
174.20
220.10
28.0
Diazepam-d5
290.12
198.10
227.10
Methadone
310.20
105.10
265.10
29.0
Diazepam-d5
290.12
198.10
227.10
Methamphetamine
150.10
65.30
91.20
9.5
Methamphetamine-d5
155.10
91.20
92.20
Midazolam
326.10
249.20
291.20
28.0
Diazepam-d5
290.12
198.10
227.10
Morphine
286.10
152.10
165.00
78.0
Morphine-d3
289.10
152.10
165.10
Naloxone
328.21
212.00
310.10
23.0
7-Amino-clonazepam-d4
290.11
226.10
254.10
Naltrexone
342.20
270.10
324.20
16.0
MDA-d5
185.10
110.20
137.10
Norbuprenorphine
414.30
187.10
340.30
99.0
Temazepam-d5
306.10
260.10
288.10
Nordiazepam
271.00
140.10
208.10
100.5
Nordiazepam-d5
276.10
165.00
213.10
Norfentanyl
233.20
55.30
84.30
16.0
MDMA-d5
199.10
135.10
165.10
Normeperidine
234.20
111.10
160.10
0.3
Temazepam-d5
306.10
260.10
288.10
Oxazepam
287.00
241.00
269.00
82.0
Oxazepam-d5
292.10
246.10
274.10
Oxycodone
316.20
241.20
298.20
22.5
Benzoylecgonine-d3
293.10
105.10
171.10
Oxymorphone
302.10
227.10
284.20
35.0
7-Amino-clonazepam-d4
290.11
226.10
254.10
PCP
244.20
86.20
159.10
84.5
Diazepam-d5
290.12
198.10
227.10
Propoxyphene
340.20
58.20
91.10
15.0
Diazepam-d5
290.12
198.10
227.10
Temazepam
301.00
255.00
283.00
36.0
Temazepam-d5
306.10
260.10
288.10
Tramadol
264.20
58.30
246.10
3.0
Temazepam-d5
306.10
260.10
288.10