243 / 658
7
0
50
100
150
200
250
300
350
400
0
50
100
150
200
250
300
350
400
Validated Method (ng/mL)
Prelude SPLC-Based Method (ng/mL)
2-Hydroxyethylflurazepam
7-Aminoclonazepam
7-Aminoflunitrazepam
a-Hydroxalprazolam
a-Hydroxytriazolam
Diazepam
Lorazepam
Nordiazepam
Oxazepam
Temazepam
R
2
= 0.9377
Figure 11. Data correlation between Prelude SPLC-based benzo-diazepines method and a previously validated
LC/MS method
Compound
Precision % RSD
Intra-assay
Inter-assay
LQC
HQC
LQC
HQC
2-Hydroxyethylflurazepam
<10
<3.3
8.9
4.2
7-Aminoclonazepam
<2.5
<2.7
2.5
2.0
7-Aminoflunitrazepam
<3.6
<3.2
3.1
2.9
7-Aminonitrazepam
<2.7
<3.6
2.4
2.7
a
-Hydroxyalprazolam
<5.8
<4.1
5.8
4.5
a
-Hydroxytriazolam
<5.9
<4.1
7.2
3.8
Alprazolam
<5.2
<2.1
3.5
2.3
Desalkyflurazepam
<5.3
<5.9
3.6
2.3
Diazepam
<2.8
<3.0
3.1
2.2
Lorazepam
<5.3
<4.5
6.7
3.3
Midazolam
<1.2
<5.4
2.8
1.6
Nordiazepam
<4.0
<4.5
5.1
2.5
Oxazepam
<3.3
<3.2
3.3
3.9
Temezepam
<5.3
<3.1
4.3
3.6
Table 5. Intra and inter-assay precision for benzodiazepines analyses