241 / 658
0
5000
10000
15000
20000
25000
30000
35000
40000
0
5000
10000
15000
20000
25000
30000
35000
40000
Validated Method (ng/mL)
Prelude SPLC-Based Method (ng/mL)
Codeine
Morphine
Hydrocodone
Hydromorphone
Oxycodone
Oxymorphone
0
500
1000
1500
2000
2500
3000
0
1000 2000 3000
Validated Method (ng/mL)
Prelude SPLC-Based Method (ng/mL)
R
2
= 0.9974
Figure 8. Data correlation between Prelude SPLC-based opiates method and a previously validated LC/MS method
Compound
Precision % RSD
Intra-assay
Inter-assay
LQC
MQC
HQC
LQC
MQC
HQC
Morphine
<7.0
<2.8
<2.3
8.3
2.5
3.3
Hydromorphone
<3.7
<1.8
<1.7
4.7
2.2
2.7
Oxymorphone
<5.9
<4.8
<4.9
9.8
4.9
9.8
Codeine
<8.2
<11
<2.2
8.2
4.8
3.0
Hydrocodone
<4.7
<3.8
<2.8
4.7
3.9
4.2
Oxycodone
<7.4
<3.9
<2.8
7.1
3.8
3.6
Table 3. Intra- and inter-assay precision for opiates analyses
Urine Lot#
% Recovery
Morphine
Hydromorphone Oxymorphone
Codeine
Hydrocodone Oxycodone
1
96.1
93.7
104
102
99.7
93.9
2
99.8
93.8
101
100
99.7
99.6
3
91.0
98.5
101
102
98.1
93.8
4
90.7
96.5
105
103
95.8
101
5
93.9
103
94.9
99.7
97.0
96.9
6
92.3
100
107
109
106
103
7
92.0
97.8
108
109
100
103
Table 4. Results of matrix effect experiment showing percent recovery of opiates in spiked urine
5