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Application Note 588
Conclusion
Using the Prelude SPLC system, high-throughput, cost-
efficient solutions were developed for forensic analysis of
opiates and benzodiazepines in urine. The methods met
industry requirements for precision, accuracy, and
robustness. Implementation of the method on a Prelude
SPLC simplified the work flow and resulted in a 40–60%
reduction of solvent usage due to the ability of the system to
utilize high efficiency, small diameter columns. The mobile
phase volumes in developed methods were approximately
3.8 mL per sample, which reduced cost of reagents and
waste disposal. Multiplexing into a single mass spectrometer
increased MS utilization and reduced overall system
hardware costs relative to two independent LC-MS systems.
The Prelude SPLC system makes multiplexing of two
different methods, with or without on-line sample prep,
possible and enabled a throughput of 480 samples in
24 hours. The implementation of methods was facilitated by
the many ease-of-use features incorporated into the system.
Acknowledgement
Thanks to Kent Johnson, Director of Toxicology at
Pacific Hospital of Long Beach, CA for providing donor
urine samples and data collected with their validated
LC-MS method.
For Forensic Toxicology use only.
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Table 6. Results of matrix effect experiment showing percent recovery of benzodiazepines in spiked urine
Urine
Lot#
% Recovery
Desalkyflura-
zepam
Diazepam
Lorazepam
Midazolam
Nordiazepam
Oxazepam
Temezepam
1
114
118
110
117
110
105
111
2
109
111
104
116
112
99.8
105
3
108
112
103
117
113
103
102
4
107
114
108
114
118
105
106
5
105
115
108
117
112
99.0
106
6
114
113
109
115
111
104
105
7
108
113
96.2
117
112
98.4
103
8
107
107
101
112
111
95.7
103
Urine
Lot#
% Recovery
2-Hydroxy-
ethylflurazepam
7-Aminoclona-
zepam
7-Aminoflunitra-
zepam
7-Aminonitra-
zepam
a
-Hydroxy-
alprazolam
a
-Hydroxy-
triazolam
Alprazolam
1
112
104
103
99.2
111
114
116
2
102
99.8
103
104
112
116
113
3
106
103
101
102
113
116
108
4
112
104
106
100
108
111
114
5
100
102
102
95.8
110
111
108
6
118
105
109
104
113
118
111
7
106
101
99.7
104
111
124
110
8
107
97.8
98.5
101
112
93.0
107
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