250 / 658
Carryover
The lowest calibrator was analyzed after the highest
calibrator. No carryover causing elevated measurements
of the drugs in the lowest calibrator was observed.
Conclusion
The developed method provides a simple, fast, and sensitive
way for forensic toxicology labs to simultaneously quantify
43 drugs of abuse, including pain management drugs, in
human urine by LC-MS/MS. The method provided LLOQ
values of 2–20 ng/mL for 42 of the 43 drugs, and was
linear to 1000 ng/mL. Minimal ion suppression and no
carryover were observed in matrix samples. At the LLOQ,
the accuracy ranged between 89.9% and 118.4%.
Method precision ranged between 1.1% and 10.9% at
low and high QC samples.
For forensic toxicology use only.
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Application Note 576