3
Thermo Scientific Poster Note
•
BioPharma_PN63946
_E 11/13S
130115_cems_tras-20kv5ps
130115_cems_tras-20kv5psi6
F:
FTMS+p NSId Fullms2 10
200
220
0
5
10
15
20
25
30
35
40
45
RelativeAbundance
215.0547 232.
130115_cems_tras-20kv5psi6
F:
FTMS+p NSId Fullms2 10
200
220
0
5
10
15
20
25
30
RelativeAbundance
215.0548 227.1
130115_cems_tras-20kv5psi60sec60mi_03
1/15/2013 8:47:17 PM
RT:
15.05 - 54.97
16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46 48 50 52 54
Time (min)
0
20
40
60
80
100
0
20
40
60
80
100
Relative Abundance
0
20
40
60
80
100
31.99
30.67
33.92 34.35
30.16
25.70
25.46
30.00
46.31
44.57
28.62
35.65
36.00
46.74
25.24
37.62
23.51
22.01
19.62
18.17
47.76
38.66 41.40
50.74 52.30
31.62
30.44
33.43
29.90
33.85
25.96
25.71
29.77
28.26
45.03
43.42
35.03
35.36
45.38
25.51
24.20
36.88
22.35
20.52
18.67
40.23
54.93
50.29
46.41
51.40
32.36
31.09
34.21 34.63
26.62
26.36
30.59
29.14
46.12
44.72
35.88
36.71
46.95
37.83
26.16
24.39
22.57
21.33
18.97
39.57
47.79 50.30
54.83
42.16
NL: 3.17E10
TIC F: FTMS + p NSI
Full ms
[150.00-1800.00] MS
130115_CEMS_Tras-
20kv5psi60sec60mi_01
_OK
NL: 3.25E10
TIC F: FTMS + p NSI
Full ms
[150.00-1800.00] MS
130115_cems_tras-
20kv5psi60sec60mi_02
NL: 3.01E10
TIC F: FTMS + p NSI
Full ms
[150.00-1800.00] MS
130115_cems_tras-
20kv5psi60sec60mi_03
130115_cems_beva-20kv5psi60sec60mi_06
1/16/2013 12:45:49 AM
RT:
15.04 - 55.08
16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46 48 50 52 54
Time (min)
0
20
40
60
80
100
0
20
40
60
80
100
Relative Abundance
0
20
40
60
80
100
29.11
28.16
31.48
27.95
25.75
22.15
25.49
32.72
42.74
41.86
24.47
33.11
43.70
34.15 36.62
21.99
54.10
53.14
51.19
37.74
49.62
20.05
45.51
18.37
29.12
28.20 31.01 31.43
22.50
26.92
25.86
32.61
25.56
42.26
41.25
32.99
43.26
33.99 36.37
22.20
54.90
53.33
51.51
20.45
49.68
47.81
37.55
18.64
16.77
29.32 31.61
28.13
22.87
26.13
25.90
32.82
42.62
41.32
24.55
33.15
43.11
34.68
54.14
53.11
51.49
36.48
22.61
49.62
47.81
20.94
37.91
18.99
17.85
NL: 3.70E10
TIC F: FTMS + p NSI
Full ms
[150.00-1800.00] MS
130115_CEMS_Beva-
20kv5psi60sec60mi_0
4
NL: 4.04E10
TIC F: FTMS + p NSI
Full ms
[150.00-1800.00] MS
130115_cems_beva-
20kv5psi60sec60mi_0
5
NL: 3.52E10
TIC F: FTMS + p NSI
Full ms
[150.00-1800.00] MS
130115_cems_beva-
20kv5psi60sec60mi_0
6
FIGURE 2. Triplicate analysis of a tryptic digest of Trastuzumab
FIGURE 3. Extracted ion electropherograms of various peptides (tryptic digest of trastuzumab)
CK
AEK
TISK
Largest tryptic peptide of the mixture :
Mw=6713.3144 Da
for 2 hours with trypsin and the obtained peptide mixture was analyzed directly after by
CESI-MS.
FIGURE 1. Triplicate analysis of a tryptic digest of Bevacizumab
CESI capable of analyzing very small (< 300 Da) and very large peptides (>8000
Da) under same experimental conditions
CESI-MS provided 100% sequence coverage for each of the tested molecules
with only 1 single digest analyzed in 1 single run
FIGURE 6. M
FIGURE 5. E
•
Be
sp
ide
•
N
130115_cems_tras-20kv5
RT:
17.93 -53.24
SM:
3
18
20
0
20
40
60
80
100
0
20
40
60
80
100
RelativeAbundance
0
20
40
60
80
100
21.37
19.66
RT:
20.03 -50.05
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Time (min)
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
RelativeAbundance
0
20
40
60
80
100
0
20
40
60
80
100
31.99
30.67
33.92
30.97
34.35
30.16
25.70
25.56
33.42
32.39
30.00
46.31
44.57
28.62
26.50
35.65
36.00
46.74
25.24
36.73
24.22
23.10
22.01
38.14
21.08
47.42
38.50
49.00
41.40
40.17
42.07 43.23
27.19
27.05
25.92
44.66
29.57
45.92
31.93
33.39
24.68
27.54
22.27
35.68
21.62
30.38
23.81
49.54
36.47
47.15
41.24
40.65
47.83
38.02 38.87
42.58 43.79
27.78
27.61
27.95
26.44
29.47
34.56
33.29
30.32
37.32
46.57
40.78
38.83
47.20
36.77
43.36
42.77
24.34
23.34
48.95
21.81
20.65
43.96
28.54
28.13
28.70
27.05
30.00
33.23
48.32
33.62
33.79
33.89
35.40 36.49
31.72 32.37
36.75 38.00
31.36
40.41 41.23
44.06
47.15
42.05
48.63
46.66
45.24
27.84
22.53
