Peptide Mapping and Analysis
Peptide mapping is a critical tool for biopharmaceuticals, and has become a widely used method for
comprehensive characterization of proteins, providing information on post-translational modifications
(PTMs), sequence, degradation products and stability. Typical peptide mapping workflows involve
enzymatic digestion followed by LC/UV, LC/MS, CE-MS or direction infusion MS analysis.
LC/UV peptide mapping workflows provide a comparative analysis that can be used to confirm the
primary structure, consistency and stability of biopharmaceutical products, and has traditionally applied
to late stage development and quality control (QC). For more in-depth protein mapping characterization,
high-resolution, accurate mass MS is needed to clearly resolve the large number of peptides and peptide
variants in such workflows, as well to provide confident assignment of peptide identifications.
The high-resolution, accurate mass of the Orbitrap family of mass spectrometers, combined with various
fragmentation capabilities and the selectivity of MS/MS, provides a superior range of MS options for in-
depth peptide mapping characterization applications. High speed automated peptide mapping can be
performed using Thermo Scientific
™
PepFinder
™
software, providing in-depth profiling of biotheraputics.
Numerous Thermo Scientific chromatography systems, columns and sample prep products offer a
complete range of solutions for all types of biopharmaceutical peptide mapping needs as demonstrated
in the following papers.
