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Conclusion
A sensitive LC-MS/MS method was developed to quantify
plasma free metanephrines in clinical research laboratories.
This method has an LLOQ of 7.2 and 18.0 pg/mL for
metanephrine and normetanephrine, respectively. Method
precision ranged from 2.0% to 10.9%. Ion-pairing
SPE was used for sample preparation, and a Hypercarb
column was used for chromatographic separation of
metanephrines.
Reference
1. He, X.; Gabler, J.; Yuan, C.; Wang, S.; Shi, Y.; Kozak, M. Quantitative
Measurement of Plasma Free Metanephrines by Ion-pairing Solid Phase
Extraction and Liquid Chromatography-Tandem Mass Spectrometry with
Porous Graphitic Carbon Column J. Chromatogr. B Analyt. Technol.
Biomed. Life Sci. 2011, 879(23), 2355-2359.
MN:
m/z 180 148
20 pg/mL
NMN:
m/z 166 134
104 pg/mL
Retention Time (min)
2.8 2.95 3.1 3.25 3.4 3.55 3.7
0
1960
Intensity
Figure 6. Representative SRM chromatograms of MN and NMN using a
processed human plasma sample