38 / 658
Thermo Fisher Scientific,
San Jose, CA USA is
ISO 9001:2008 Certified.
Acknowledgements
Thanks to Graham Carter (DEQAS) for permission to
quote DEQAS data.
References
1. El-Kouhry, J.M.; Reineks, E.Z. and Wang, S.
Clin.
Biochem.
2011
,
44
, 66-76.
2. Leaver, N.; Chihoho, B.; Robinson, S.;
Quantitative
Analysis of 25-OH Vitamin D
3
/D
2
Comparing 1D
Chromatography, 2D Chromatography and Automated
Online Separation;
Application Note 526,
AN63358_E 11/10S; Thermo Fisher Scientific;
San Jose, CA,
2010
.
3. Couchman, L.; Benton, C.M. and Moniz, C.F.
Clin.
Chim. Acta
2012
,
413
, 1239-1243.
4. Vitamin D External Quality Assessment Scheme
(DEQAS) Distribution October 2011 Summary Report.
London: Charing Cross Hospital;
2011
.
Conclusion
The Versette automated liquid handling system reduced
the time required to prepare 96 samples from two hours
to less than 20 minutes – a dramatic reduction of 80%.
In addition, the Versette system reduced the number of
manual pipetting steps from as many as 864 to none.
The workflow minimized manual errors, increased method
precision, and reduced the risk of repetitive strain injury
in research laboratories.
Using multiplexing on the Transcend TLX-2 and an
analytical column using a pentafluorophenyl stationary
phase, the method resolved 3-
epi
-25OHD
3
, an interferent
in most LC-MS/MS 25OHD
3
methods, without significantly
decreasing chromatographic throughput.
The same basic workflow employing automated liquid
handling and automated online sample preparation can be
used by research laboratories for the analysis of other
compounds including mass spectrometric immunoassay-
selective (MSIA) assays to measure parathyroid hormone
(PTH) and vitamin D-binding protein.
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