41 / 658
3
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6
Time (min)
0
10
20
30
40
50
60
70
80
90
100
25-Hydroxyvitamin D
2
RT: 1.67
RT:
0.00 - 3.60
SM:
5G
RT:
0.00 - 3.60
SM:
5G
RT:
0.00 - 3.60
SM:
5G
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6
Time (min)
0
10
20
30
40
50
60
70
80
90
100
25-Hydroxyvitamin D
3
RT: 1.64
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6
Time (min)
0
10
20
30
40
50
60
70
80
90
100
d6 25-Hydroxyvitamin D
3
RT: 1.63
NL: 1.73E5
m/z
=
383.32969–383.33199
F: FTMS + p APCI
corona
[350.00–425.00] MS
ICIS Cal4
NL: 1.10E5
m/z
=
395.32965–395.33203
F: FTMS + p APCI
corona
[350.00–425.00] MS
ICIS Cal4
NL: 8.39E6
m/z
=
389.36733–389.36967
F: FTMS + p APCI
corona
[350.00–425.00] MS
ICIS Cal4
Figure 5. Calibration curve of 25-hydroxyvitamin D
3
in BSA
Figure 4. Calibration curve of 25-hydroxyvitamin D
2
in BSA
Figure 3. Chromatograms at LLOQ of 2 ng/mL with 50 ng/mL internal standard
fig4
25-Hydroxyvitamin D
2
Y= -0.000801351+0.00392862*X R^2 = 0.9940 W: 1/X
0
20
40
60
80
100
ng/mL
0.0
0.1
0.2
0.3
0.4
fig5
25-Hydroxyvitamin D
3
Y= 0.00183722+0.0059339*X R^2 = 0.9989 W: 1/X
0
20
40
60
80
100
ng/mL
0.0
0.1
0.2
0.3
0.4
0.5
0.6