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Application Note 567
Thermo Fisher Scientific,
San Jose, CA USA is ISO Certified.
www.thermoscientific.com©2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. This information is
presented as an example of the capabilities of Thermo Fisher Scientific Inc. products. It is not intended to encourage use of these products in any manners that
might infringe the intellectual property rights of others. Specifications, terms and pricing are subject to change. Not all products are available in all countries.
Please consult your local sales representative for details.
AN63534_E 06/12S
Table 2. 20 ng/mL serum injection replicates
25-hydroxyvitamin D
2
2 ng Area
Replicate 1
134195
Replicate 2
162585
Replicate 3
148309
Mean
148363
SD
14195.1
%CV
9.6
Table 1. 2 ng/mL replicate LLOQ injections
25-hydroxyvitamin D
3
2 ng Area
Replicate 1
201766
Replicate 2
242186
Replicate 3
212094
Mean
218682
SD
20999.9
%CV
9.6
D
2
20 ng Serum
Area
Replicate 1
4464244
Replicate 2
3757594
Replicate 3
4544819
Replicate 4
4332109
Replicate 5
3857037
Replicate 6
4581097
Replicate 7
5148234
Replicate 8
4704084
Replicate 9
4319873
Replicate 10
4175023
Mean
4388411
SD
405245.1
%CV
9.2
D
3
20 ng Serum
Area
Replicate 1
11759664
Replicate 2
10759647
Replicate 3
10886536
Replicate 4
10825748
Replicate 5
12543252
Replicate 6
12223745
Replicate 7
11278373
Replicate 8
11445949
Replicate 9
12537176
Replicate 10
11033701
Mean
11529379
SD
698829.3
%CV
6.1
Conclusion
An Exactive Plus high-resolution Orbitrap mass
spectrometer with TurboFlow automated on-line sample
extraction technology provides reliable detection for
clinical researchers of 25-hydroxyvitamin D
2
and
25-hydroxyvitamin D
3
in serum.
In addition, the Exactive Plus MS offers higher resolving
power and easier initial method setup than triple
quadrupole mass spectrometers. Throughput can be
increased to a sample every 3.7 minutes by using a
Transcend TLX-2 multiplexed UHPLC system or a sample
every 1.9 minutes with a Transcend TLX-4 system.
For Research Use Only. Not for use in diagnostic
procedures.
Thermo Fisher Scientific (Bremen) GmbH
Management System Registered to ISO 9001:2008