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Conclusion
A robust 10-minute method for the quantification of
mevalonate with a dynamic range of 2.5 - 250 ng/mL
plasma has been developed for clinical research using
fast SPE purification and the LTQ linear ion trap mass
spectrometer.
Figure 5. Ion chromatograms of plasma sample containing 24 ng/mL
Mevalonate
Y = 0.00317513+0.00579147*X R^2 = 0.9993 W: 1/X
0
50
100
150
200
250
ng/mL
0.0
0.5
1.0
1.5
Area Ratio
Figure 3. Calibration curve of mevalonate
Mevalonate
Y= 0.00317513+0.00579147*X R^2 = 0.9993 W: 1/X
0
5
10
15
20
25
30
ng/mL
0.00
0.05
0.10
0.15
0.20
0.25
0.30
Area Ratio
Figure 4. Zoom on low calibration points
Mevalonate
Mevalonate-D
7
1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0
Time (min)
0
20
40
60
80
100
RelativeAbundance
0
20
40
60
80
100
RT: 5.12
RT: 5.09