2
Consumables
Part Number
Column:
TraceGOLD TG-5MS 30 m × 0.25 mm × 0.25 µm 26098-1420
Septum:
BTO coated 11 mm center guide (50/pk)
31303233
Liner:
Splitless liner with single taper 78.5 × 4 × 6.3 mm 453A1925
Column ferrules:
Graphite ferrule for 0.1–0.32 mm i.d. columns 10/pk
290GA139
Injection syringe:
10 µL syringe FN 50 mm T Gauge 26, cone tip
36500525
Vials and closures:
Thermo Scientific 9 mm Wide Opening Screw Thread 60180-599
Vials Convenience Kit, 2 mL Clear Glass Vial with ID Patch,
Blue Closure with PTFE/Blue Silicone Septa
Solutions
A working standard solution of 20 µg/mL of EPA 8141 was prepared in acetone. The stock solution was obtained
commercially at a concentration of 1000 µg/mL.
Separation Conditions
Part Number
Instrumentation:
TRACE 1310 mainframe 230 V GC
14800302
Carrier gas:
Helium
Split flow:
50 mL/min
Column flow:
1.2 mL/min, constant flow
Oven temperature:
40 °C (1 min), 12 °C/min, 280 °C (10 min)
Injector type:
TRACE 1310 SSL Injector module
29903010
Injector mode:
Splitless
Injection details:
Splitless (1 min)
Injector temperature:
220 °C
Detector details:
TRACE 1310 FID module 29903001
FID parameters:
Temperature:
280 °C
Air flow:
350 mL/min
Hydrogen flow:
35 mL/min
Nitrogen makeup flow:
30 mL/min
Injector Conditions
Instrumentation:
Thermo Scientific AS1300 Autosampler
Injection Volume:
1 µL
Wash solvent:
Acetone/hexane (1:1 v/v)
Data Processing
Software:
Thermo Scientific
TM
Chrom-Card
TM
data system
Results
Figure 1 shows the TIC chromatogram for 22 OPPs at 20 µg/µL using a TraceGOLD TG-5MS
column and a standard, deactivated, splitless quartz liner for the TRACE 1310 GC instrument.
Table 1 shows the peak identification of the OPPs according to their retention times. Table 1
includes the reproducibility data for ten injections. The stationary phase of the TG-5MS GC
column, in combination with the deactivated splitless liner, provides excellent performance due to
minimal interaction of active compounds with active sites on the column, the glass wall of the liner,
or the deactivated quartz wool. This minimizes peak tailing of the OPPs and gives highly
symmetrical peak shapes. The combination of a TG-5MS GC column, the deactivated liner, and the
TRACE 1310 GC gave excellent injection reproducibility of between 1.7% and 3.4% for the 22
OPPs tested (Table 2).
