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Figure 4: 20 µg/dL Cortisone Standard with deuterated internal standard (d3-cortisol)
Figure 5: Urine cortisol calibration curve
Figure 6: Urine cortisone calibration curve
Cortisol
Cortisone
Intra-assay
Inter-assay
Intra-assay
Inter-assay
Urine Low Pool (n=20)
14.9%
N/A
6.9%
N/A
Urine QC Pool 2 (n=20)
8.6%
N/A
7.6%
N/A
Urine QC Pool 3 (n=20)
8.3%
N/A
7.1%
N/A
Urine QC Pool 1 (n=10)
N/A
20.2%
N/A
14.1%
Urine QC Pool 2 (n=10)
N/A
6.0%
N/A
8.7%
Urine QC Pool 3 (n=10)
N/A
7.5%
N/A
7.2%
Urine QC Pool 4 (n=10)
N/A
6.1%
N/A
6.2%
Table 3: Intra- and inter- assay precision