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Figure 1: Data for 1,25D2 (Transition 411→151)
D2 151
Y=0.0475068+0.0295773*X R^2=0.9999 W:Equal
0 50 100 150 200 250 300 350
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
11.0
AreaRatio
Concentration (pg/mL)
1,25D2 Measured (pg/mL) Specified (pg/mL)
Cal1 34.8 33
Cal2 349.8 350
Ctrl1 94.3 63-105
Ctrl2 311.6 203-348
1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8
0
10
20
30
40
50
60
70
80
90
100 SN: 108RMS
Retention Time (min)
1,25D3 Measured (pg/mL) Specified (pg/mL)
Cal1 21.7 26
Cal2 250.4 250
Ctrl1 59.9 49-81
Ctrl2 200.8 146-244
Cal1
D3 151
Y= -0.0997963+0.0260387*X R^2=0.9991 W:Equal
0 20 40 60 80 100 120 140 160 180 200 220 240 260
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
AreaRatio
Concentration (pg/mL)
Calibration Curve (Cal1, Cal2)
1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8
0
10
20
30
40
50
60
70
80
90
100 SN: 62 RMS
Retention Time (min)
Cal1
Figure 2: Data for 1,25D3 (Transition 399→151)
Calibration Curve (Cal1, Cal2)