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0
20
40
60
80
100
Relative Abundance
3.58
3.27
4.26
4.01
4.28
3.75 3.97
3.31
4.48
TurboFlow method
Figure 4. XICs for 25-OH vitamin D
3
by (A) TurboFlow method and (B) 1D LC-MS/MS analysis of a sample at normal levels of analyte
(83 nmol/L).
A
0
20
40
60
80
100
Relative Abundance
3.52
3.66
4.58
4.61
3.68
3.73
5.17
5.16
4.48
3.80
5.21
4.77
3.23
3.00
4.37
1D LC-MS/MS
B