AI-64310-CRFT

52 / 658

Part of Thermo Fisher Scientific
Conclusion
We have developed a novel 10-min LC-MS/MS method
for quantitation of E1 and E2 in serum using TurboFlow
technology for clinical research laboratories. This method
is fast and analytically sensitive and sample preparation
effort is significantly reduced. The Accucore HPLC column
was used for analytical LC separation because of its
superior performance. The lower limit of quantitation was
3.8 pg/mL for estrone and 3.7 pg/mL for estradiol. This
method was linear from 3.8 to 1000.9 pg/mL for estrone
and 3.7 to 993.1 pg/mL for estradiol with accuracy from
95.5% to 103.2% for estrone and from 92.7% to 112.3%
for estradiol, respectively. Inter-assay and intra-assay CV
for estrone and estradiol at low and high concentration
levels in both spiked charcoal stripped serum and pooled
human plasma ranged from 3.5% to 18.0%.
Legal Notices: ©2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
This information is presented as an example of the capabilities of Thermo Fisher Scientific Inc. products. It is not intended to encourage use of these products in any
manners that might infringe the intellectual property rights of others. Specifications, terms and pricing are subject to change. Not all products are available in all
countries. Please consult your local sales representative for details.
Thermo Fisher Scientific,
San Jose, CA USA is ISO Certified.
In addition to these
offices, Thermo Fisher
Scientific maintains
a network of represen­
tative organizations
throughout the world.
Africa-Other
+27 11 570 1840
Australia
+61 3 9757 4300
Austria
+43 1 333 50 34 0
Belgium
+32 53 73 42 41
Canada
+1 800 530 8447
China
+86 10 8419 3588
Denmark
+45 70 23 62 60
Europe-Other
+43 1 333 50 34 0
Finland/Norway/
Sweden
+46 8 556 468 00
France
+33 1 60 92 48 00
Germany
+49 6103 408 1014
India
+91 22 6742 9434
Italy
+39 02 950 591
Japan
+81 45 453 9100
Latin America
+1 561 688 8700
Middle East
+43 1 333 50 34 0
Netherlands
+31 76 579 55 55
New Zealand
+64 9 980 6700
Russia/CIS
+43 1 333 50 34 0
South Africa
+27 11 570 1840
Spain
+34 914 845 965
Switzerland
+41 61 716 77 00
UK
+44 1442 233555
USA
+1 800 532 4752
AN63519_E 01/12S
Reference
1. Xiang He and Marta Kozak, Fast and Sensitive LC-APCI-MS/MS
Quantitative Analysis of Estrone and Estradiol in Serum without Chemical
Derivatization, Thermo Fisher Scientific Application Note 530.
For Research Use Only.
Not for use in diagnostic procedures.