51 / 658
Carryover
CSS was spiked with E1 and E2 to create a high-level
sample (>500 pg/mL) and a low-level sample (8 pg/mL).
The low-level sample was injected first (Low1) for
LC-MS/MS analysis followed by the injection of the
high-level sample (High). Immediately afterward, another
low-level sample was injected (Low2). No carryover was
observed by testing the spiked CSS samples with Low1
(9.9 pg/mL)-High (556.0 pg/mL)-Low2 (9.1 pg/mL) for
E1 and Low1 (10.0 pg/mL)-High (582.5 pg/mL)-Low2
(8.9 pg/mL) for E2.
Data examples of clinical research samples
Figures 6 and 7 show the SRM chromatograms of E1
and E2 in two individual plasma samples.
Figure 6. SRM chromatograms of E1 and E2 in human plasma sample 1 (female)
Figure 7. SRM chromatograms of E1 and E2 in human plasma sample 2 (male)
RetentionTime (min)
6.2
6.5
6.8
7.1
7.4
7.7
8.0
0
198
E1:
m/z
269
145
11.9 pg/mL
E2:
m/z
271 183
3.1 pg/mL
Intensity
RetentionTime (min)
6.2
6.5
6.8
7.1
7.4
7.7
8.0
0
100
E1:
m/z
269
145
6.9 pg/mL
E2:
m/z
271 183
5.1 pg/mL
Intensity