498 / 658
4
3
4
5
6
Time (min)
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
RT: 3.06
RT: 4.28
3.06
RT: 4.41
RT: 5.50
RT: 5.63
6
7
8
9
Time (min)
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
RT: 5.98
RT: 6.11
RT: 6.45
RT: 7.17
10
11
12
Time (min)
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
RT: 9.60
RT: 10.14
RT: 11.30
RT: 11.35
RT: 11.95
RT: 12.07
Theobromine
Theophylline
Dyphylline
Caffeine
Chlorothiazide
Cromolyn-Na
Hydrochlorothiazide
Pemoline (not found)
Pentoxifylline
Dexamethasone (not found)
Boldenone
Ketoprofen
Indomethacin
Diclofenac
Flufenamic Acid
Meclofenamic Acid
Figure 4: Reconstructed ion chromatograms, using the product ions listed in Table 1 (RIC column), from the analysis of a 10 µL injection of the 50 pg/µL
standard in horse urine
Figure 5: Representative calibration curves from standards prepared
in solvent
Figure 6: Representative calibration curves from standards prepared
in horse urine
Theobromine
Y = 18.5962+131.036*X R^2 = 0.9986
0
20
40
60
80
100
0
2000
4000
6000
8000
10000
12000
14000
Area
Theophylline
Y = -79.8728+249.537*X R^2 = 0.9998
0
20
40
60
80
100
0
5000
10000
15000
20000
25000
Area
Dyphilline
Y = -399.929+957.285*X R^2 = 0.9997
0
20
40
60
80
100
0
20000
40000
60000
80000
100000
Caffeine
Y = -604.499+521.811*X R^2 = 0.9991
0
20
40
60
80
100
0
10000
20000
30000
40000
50000
Area
Theophylline
Y = -233.655+246.415*X R^2 = 0.9994
0
200
400
600
0
50000
100000
150000
Area
Caffeine
Y = 1103.78+772.157*X R^2 = 0.9996
0
200
400
600
0
100000
200000
300000
400000
500000
Area
Theobromine
Y = 4568.38+224.452*X R^2 = 0.9974
0
1000
2000
3000
4000
0
200000
400000
600000
800000
Area
Dyphilline
Y = 9455.91+862.639*X R^2 = 0.9993
0
200
400
600
0
100000
200000
300000
400000
500000
600000