414 / 658
6
LC/MS/MS Research Method for 14 Antidepressants Utilizing Dried Blood Spots
ts were performed for system verification on
oxepin, imipramine, fluvoxamine,
m, nortriptyline, desipramine, venlafaxine,
day and intra-day accuracy and precision
te. A summary of the results is shown in
at were less than 15.0% for all compounds
0.0%. Additionally, accuracy was ±15.0% of
ion coefficient values for all compounds
y throughout all concentrations and analytes.
lectivity criteria, as well as benchtop and
all above 90.0% including matrix effects.
shown in Figure 4. Examples of the
recision summary
Paroxetine Citalopram Nortriptyline Desipramine Venlafaxine Sertaline Duloxetine Bupropion
18.2
18.9
18.4
19.4
21.4
19.1
18.9
17.3
9.28
5.38
7.88
3.20
7.00
4.75
5.70
13.70
115
115
116
111
114
17
117
118
4.18
4.73
5.64
0.91
4.00
6.00
1.91
2.44
394
391
409
391
401
415
389
375
3.68
2.89
7.58
2.95
5.42
9.16
2.42
1.27
ms at the LOQ
2
3
0
1000
2000
3000
0
1
2
3
0
325
650
975
1300
0
1
2
3
2
3
0
325
650
0
1
2
3
2
3
0
750
1500
0
1
2
3
Doxepin
impiramine
omipramine
Fluoxetine
italopram
Nortriptyline
Venlafaxine
Sertraline
Bupropion
2
3
2
3
FIGURE 5. Representative calibration curves
Conclusion
•
An LC-MC/MS research method has been successfully developed and verified for the
quantification of 14 antidepressant drugs collected from dried blood spots using a Prelude SPLC
system and a TSQ Endura triple quadrupole mass spectrometer.
•
Online sample cleanup of the matrix resulting from dried blood spot collection reduced the
complexity of the LC-MS/MS workflow.
•
Due to the use of online sample preparation, this research method is more accurate, easier to
perform, takes less time, is more robust, and is less costly then traditional offline sample
preparation such as SPE plates.
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