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Y = 1368.24+1286.83*X R^2 = 0.9955 W : 1/X

0

500

1000

ng /mL

0

200000

400000

600000

800000

1000000

1200000

1400000

Area

THC-COOH_neg

Figure 3. Calibration curve of THC-COOH in negative ionization mode

RT:

2.00 - 4.00

SM:

3G

2.0

2.2

2.4

2.6

2.8

3.0

3.2

3.4

3.6

3.8

4.0

Time (min)

5

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

95

100

THC-COOH neg

RT: 3.07

Figure 4. Ion chromatogram of urine sample containing 225 ng/mL. Sample was hydrolyzed and diluted 1:10 before the analysis.

[M-H] -