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6.5
7.0
7.5
8.0
8.5
9.0
9.5
Time (min)
0
20
40
60
80
100
RelativeAbundance
0
RelativeAbundance
0
20
40
60
80
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RelativeAbundance
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
JWH-073-OH (not detected)
JWH-018-OH (5.71 ng/mL)
JWH-073-COOH (11.6 ng/mL)
JWH-018-COOH (9.38 ng/mL)
Figure 5. SRM chromatogram of self-confessed human in vivo sample. JWH-073-N-(4-hydroxybutyl), a
compound validated in this assay, is not seen in this sample. The unidentified peak in the JWH-073-OH
channel is JWH-073-N-(3-hydroxybutyl), a major metabolite not known at the time of this validation.
Conclusion
A simple dilute and shoot method for the analysis of syn-
thetic cannabinoid metabolites in urine was developed for
forensic toxicology use. Since analysis of these compounds
is relatively new to forensic applications, cut-off values
have not been established. The current method has an
LOQ of 2 ng/mL for all compounds. Based on published
research, using an SPE or liquid/liquid extraction process-
ing method will lower the current LOQ to 0.2 ng/mL, if
required.