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Methamphetamine and phentermine (an anti-obesity
drug) are structural isomers with identical molecular
masses and similar fragments. To avoid false positives,
they must be separated chromatographically. As seen in
Figure 3, these two compounds are well-resolved and will
not interfere with each other.
Conclusion
A method with simple dilute-and-shoot sample prepara-
tion for the confirmation of amphetamines in urine was
developed. This method is suitable for SAMHSA-man-
dated workplace drug testing, meeting cutoff and speci-
ficity requirements within a 4.5-minute run. The sample
processing method also enables all SAMHSA panels to be
processed at once.
Time (min)
2.5 2.6 2.7 2.8 2.9 3.0 3.1 3.2
Amphetamine
Methamphetamine
MDMA MDEA
MDA
2.6 2.7 2.8 2.9 3.0 3.1 3.2
Methamphetamine Phentermine
NH2
CH3
CH3
NH
CH3
CH3
Figure 2. SRM chromatogram of amphetamine, methamphetamine, MDA, MDMA and MDEA in urine at their respective LOQs
Figure 3. SRM chromatogram showing excellent resolution between structural isomers methamphet-
amine and phentermine