264 / 658
Table 1. Method summary for quantitation of amphetamines in urine
Compound
LOQ
ULOQ
SAMHSA Cutoff
Amphetamine
10 ng/mL
5000 ng/mL
250 ng/mL
Methamphetamine
5 ng/mL
5000 ng/mL
250 ng/mL
MDA
20 ng/mL
5000 ng/mL
250 ng/mL
MDMA
5 ng/mL
5000 ng/mL
250 ng/mL
MDEA
5 ng/mL
5000 ng/mL
250 ng/mL
Phentermine Not quantitated, but chromatographically well-separated
from isomeric methamphetamine.
Total run time: 4.5 minutes
Table 2. %CV/%Bias for QCs analyzed during validation of amphetamines in urine
Compound
LQC (10 ng/mL)
MQC (100 ng/mL)
HQC (500 ng/mL)
Amphetamine
10.9/-2.24
4.45/6.39
2.56/0.431
Methamphetamine
7.03/0.420
3.02/7.78
4.26/1.67
MDA
NA
5.97/3.46
4.17/-0.196
MDMA
5.88/0.737
3.31/7.88
4.95/3.45
MDEA
4.51/3.35
2.96/8.20
4.34/2.54
NA: LQC concentration is below LOQ for MDA; data not reported.