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AN63497 _E 11/11S
Table 2. Statistical results for HRAM LC/MS quantitation of diazepam in urine
Table 2 [Diaz pam, SIM]
Specified Amount
(ng /mL )
Mean Calculated Amt.
(ng/mL)
%Accuracy
%CV
0.0125
0.0113
90.1
6.0
0.0250
0.0236
94.3
7.9
0.0625
0.0610
97.6
1.4
0.1250
0.127
101.9
2.9
0.250
0.273
109.1
1.7
2.50
2.65
105.9
1.0
25.0
25.5
102.0
1.7
125.0
123.5
98.8
0.8
250.0
250.8
100.3
1.2
spectrometer using multiplexed SIM are comparable
to those observed on triple stage quadrupole mass
spectrometers in SRM mode.
an R
2
regression value of 0.9998 using 1/x weighting.
Table 2 presents the statistical results for the HRAM
quantification of diazepam. The quantitative accuracy
and precision values obtained by the Q Exactive mass
Conclusion
The Q Exactive HRAM LC/MS system is a powerful
and flexible instrument that can provide both sample
identification and quantitative information for forensic
toxicology with a single sample analysis. By using the
method of multiplexed SIM, eight benzodiazepines in
urine were quantified with LLOQs at the pg/mL level
and with linear dynamic ranges of 3 to 4 orders of
magnitude.
For Forensic Toxicology Use Only