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Application Note
571
Analyte (40 ng/mL)
Mean Measured (ng/mL), n=9
Accuracy (%) n=9
Precision (%) n=9
Amitriptyline
47.2
118.1
7.2
Bromazepam
33.7
84.3
18.0
Clobazam
42.8
107.0
15.0
Clomipramine
41.7
104.2
12.9
Clonazepam
41.4
103.4
13.4
Clozapine
38.6
96.4
9.0
Diazepam
37.2
93.1
8.8
Dothiepin
38.3
95.8
8.1
Doxepin
41.2
102.9
18.5
Flunitrazepam
34.8
87.0
7.7
Imipramine
37.4
93.4
6.5
Levetiracetam
40.0
100.1
7.7
Lamotrigine
37.2
93.0
18.2
Nitrazepam
38.9
97.3
7.0
Nortriptyline
36.2
90.5
6.2
Oxazepam
35.3
88.2
7.2
Perhexilline
42.8
106.9
9.6
Temazepam
36.1
90.3
9.1
Trimipramine
35.9
89.8
7.9
Table 7. Accuracy and precision summary for analysis of 19 drugs in 9 individual human-donor plasma samples
Carryover
The lowest calibrator was analyzed after the highest
calibrator, and we did not observe any carryover causing
elevated measurements of the drugs in the lowest calibrator.
Conclusion
We have developed a simple, fast, and sensitive LC-MS/MS
clinical research method for simultaneously quantitation
of 19 drugs in human plasma. The method had LLOQ
values of 4–20 ng/mL for all 19 drugs and was linear to
400 ng/mL. Ion suppression was not observed in matrix
samples. Accuracy and precision of the method were
successfully accessed in both CSS and human plasma
samples.
For Research Use Only. Not for use in diagnostic procedures.