257 / 658
7
Accuracy and Precision
Accuracy and precision were first assessed with CSS
spiked at concentrations of 40 and 200 ng/mL (Table 6).
Overall accuracy ranged between 82.4% and 111.3%.
Inter- and intra-batch precision (coefficient of variation)
values at low (40 ng/mL) and high (200 ng/mL)
concentrations varied between 1.4% and 13.5%.
Accuracy and intra-batch precision were also assessed in
the 9 individual human-donor plasma samples spiked with
40 ng/mL drugs. The results were satisfactory (Table 7).
Table 6. Accuracy and precision summary for analysis of 19 drugs
in CSS
Analyte
40 ng/mL
200 ng/mL
Precision
Accuracy
Precision
Accuracy
Intra1 (%)
n=5
Intra2 (%)
n=5
Intra3 (%)
n=5
Inter (%)
n=15
Inter (%)
n=15
Intra1 (%)
n=5
Intra2 (%)
n=5
Intra3 (%)
n=5
Inter (%)
n=15
Inter (%)
n=15
Amitriptyline
8.5
10.4
11.5
9.7
87.8
4.6
3.8
9.7
6.3
100.7
Bromazepam
10.1
2.9
3.8
6.9
89.9
3.1
4.0
2.1
3.3
104.2
Clobazam
2.5
3.4
8.6
5.1
90.6
5.5
4.0
4.3
4.6
101.7
Clomipramine
8.3
8.1
6.4
8.0
106.3
3.2
3.1
3.1
4.8
109.4
Clonazepam
3.6
6.4
6.1
5.2
101.4
5.6
2.1
3.3
4.4
107.4
Clozapine
5.7
3.4
5.1
5.4
96.5
4.4
4.3
2.4
3.6
111.3
Diazepam
4.9
6.9
5.9
5.9
88.8
2.7
4.7
3.6
3.6
101.7
Dothiepin
3.7
8.9
5.4
6.1
99.5
4.2
2.5
4.0
4.9
108.2
Doxepin
5.8
10.8
11.9
10.0
96.4
4.5
4.5
2.9
4.5
108.8
Flunitrazepam
1.4
7.0
4.2
5.1
82.4
4.7
4.2
4.3
4.5
100.8
Imipramine
3.1
2.9
2.0
2.8
87.0
1.6
3.1
3.2
2.9
102.2
Lamotrigine
7.0
5.2
8.9
7.2
96.9
3.9
2.5
3.4
3.8
105.8
Levetiracetam 10.9
3.9
9.5
8.3
99.1
5.4
3.0
8.9
5.9
107.8
Nitrazepam
3.8
4.1
6.0
5.2
85.1
5.7
3.8
5.4
4.7
97.3
Nortriptyline
6.9
4.9
4.6
5.2
97.7
2.3
3.9
4.3
3.9
110.5
Oxazepam
8.3
5.5
9.2
7.6
96.5
5.0
7.1
1.7
5.2
106.3
Perhexilline
8.0
12.7
12.7
13.5
86.5
2.2
1.9
6.9
4.4
107.7
Temazepam
7.7
5.5
3.7
6.1
95.3
2.7
2.5
4.8
3.4
104.7
Trimipramine
3.6
3.0
6.1
4.1
89.0
2.9
3.7
3.9
3.7
103.4