13 / 658
Figure 3. Chromatogram of 5 pg/mL calibrator for estradiol in CSS showing quantifier and confirming ion with passing ion ratio
3
Figure 2. Chromatogram of 1 pg/mL calibrator for estradiol in CSS showing quantifier and confirming ion with passing ion ratio
5.6
5.7
5.8
RT (min)
m/z:
171.200
5.9
6.0
Relative Abundance
Estradiol RT: 5.85 | CSS_1pgmL_03
RT: 5.85
AA: 8128.86
SN: 41.03
6.1
Quan Peak
Apex RT: 5.85
Area: 8129
Apex RT: 5.85
Area: 3126
5.6
5.7
5.8
RT (min)
m/z:
156.200
29.25% – 48.75% 156.200/171.200 = 38.45%
5.9
6.0
Relative Abundance
RT: 5.85 | CSS_1pgmL_03
RT: 5.85
AA: 3125.86
SN: 43.16
6.1
Con rming Ions
5.6
5.7
5.8
RT (min)
m/z:
171.200
5.9
6.0
Relative Abundance
Estradiol RT: 5.85 | CSS_5pgmL_01
RT: 5.85
AA: 22722.19
SN: 113.55
6.1
Quan Peak
Apex RT: 5.85
Area: 22722
Apex RT: 5.85
Area: 8146
5.6
5.7
5.8
RT (min)
m/z:
156.200
23.83% – 39.71% 156.200/171.200 = 35.85%
5.9
6.0
Relative Abundance
RT: 5.85 | CSS_5pgmL_01
RT: 5.85
AA: 8145.70
SN: 108.57
6.1
Con rming Ions
Table 4. Results of mixing test showing recovery of estradiol in a
sample of human plasma diluted 1-, 2-, and 4-fold
Sample
Mean Conc
(pg/mL)
% RSD
Mean
% Rec
Plasma
20.6
1.1
–
2-fold dilution
9.67
1.7
94.0
4-fold dilution
4.34
3.7
84.4