AI-64310-CRFT

18 / 658

Figure 4. Chromatogram of testosterone (A) quantifier and (B) qualifier peaks in
sample from a 4-year-old donor at concentration of 6.29 pg/mL
Figure 5. Chromatogram of testosterone (A) quantifier and (B) qualifier peaks in
sample from a 6-year-old donor at concentration of 33.2 pg/mL
Conclusion
Using the TSQ Quantiva mass spectrometer, an analytically
sensitive and robust method was evaluated for the research
analysis of low testosterone concentrations in human
plasma. The LOQ of 5 pg/mL is lower than that reported
with other research methods. Precision was better than
5% RSD, and the ion ratios easily met industry standard
criteria. The data show the TSQ Quantiva mass
spectrometer has excellent analytical sensitivity and
robustness to facilitate research.
For research use only. Not for use in diagnostic procedures.
Application Note 594
A B
m/z: 97.300 m/z: 109.300
73.62% - 110.43% 109.300/97.300 = 77.59%
A B
m/z: 97.300 m/z: 109.300
73.62% - 110.43% 109.300/97.300 = 90.95%
AN63939_E 12/13S
Africa +43 1 333 50 34 0
Australia +61 3 9757 4300
Austria +43 810 282 206
Belgium +32 53 73 42 41
Canada +1 800 530 8447
China 800 810 5118 (free call domestic)
400 650 5118
Denmark +45 70 23 62 60
Europe-Other +43 1 333 50 34 0
Finland +358 9 3291 0200
France +33 1 60 92 48 00
Germany +49 6103 408 1014
India +91 22 6742 9494
Italy +39 02 950 591
Japan +81 45 453 9100
Latin America +1 561 688 8700
Middle East +43 1 333 50 34 0
Netherlands +31 76 579 55 55
New Zealand +64 9 980 6700
Norway +46 8 556 468 00
Russia/CIS +43 1 333 50 34 0
Singapore +65 6289 1190
Spain +34 914 845 965
Sweden +46 8 556 468 00
Switzerland +41 61 716 77 00
UK +44 1442 233555
USA +1 800 532 4752
©2013 Thermo Fisher Scientific Inc. All rights reserved. ISO is a trademark of the International Standards Organization.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries. This information is presented as an
example of the capabilities of Thermo Fisher Scientific products. It is not intended to encourage use of these products in any
manners that might infringe the intellectual property rights of others. Specifications, terms and pricing are subject to change.
Not all products are available in all countries. Please consult your local sales representative for details.
Thermo Fisher Scientific,
San Jose, CA USA is
ISO 9001:2008 Certified.