14 / 658
Conclusion
• This clinical research method was able to reach the
desired limit of quantitation of 1 pg/mL in human
plasma.
• The method shows excellent precision, accuracy, and
ion ratio confirmation over the entire calibration range
of 1 to 1000 pg/mL.
• No matrix effects were observed.
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Figure 4. Chromatogram of estradiol in donor plasma at 22.6 pg/mL showing quantifier and confirming ion with passing ion ratio
5.6
5.7
5.8
RT (min)
m/z:
171.200
5.9
6.0
Relative Abundance
Estradiol RT: 5.84 | HS_HS_01
RT: 5.84
AA: 78375.98
SN: 182.98
6.1
Quan Peak
Apex RT: 5.84
Area: 78376
Apex RT: 5.85
Area: 8146
5.6
5.7
5.8
RT (min)
m/z
: 156.200
23.83% – 39.71% 156.200/171.200 = 33.31%
5.9
6.0
Relative Abundance
RT: 5.84 | HS_HS_01
RT: 5.84
AA: 26103.53
SN: 177.30
6.1
Con rming Ions