128 / 658
Application Note 603
Table 3. QC and unknowns
Conclusion
A high-throughput, cost-efficient research method was
developed for the precise and accurate measurement of
immunosuppressant drugs in dried blood spots using a
TSQ Endura triple quadrupole mass spectrometer. This
method met analytical laboratory precision and accuracy
criteria for 3 mm dried blood spots.
For research use only. Not for use in diagnostic procedures.
AN64053-EN 0714S
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Tacrolimus
Sirolimus
Cyclosporin A
Spiked
(ng/mL)
Measured
[average ± st dev]
(ng/mL)
Spiked
(ng/mL)
Measured
[average ± st dev]
(ng/mL)
Spiked
(ng/mL)
Measured
[average ± st dev]
(ng/mL)
Low Control
6.0
6.2 ± 0.2
3.6
3.2 ± 0.3
76
74 ± 2
Mid Control
12
13.1 ± 0.5
9.7
11.0 ± 0.4
199
175 ± 3
High Control
22
21.2 ± 2.8
17.4
17.6 ± 4.2
311
276 ± 9
Subject 1
10
11.0 ± 1.0
5.3
6.2 ± 0.5
0
< LOQ
Subject 2
4.2
4.9 ± 0.4
0
< LOQ
0
< LOQ
Subject 3
0
< LOQ
2.1
3.0 ± 0.9
59
57 ± 3
ISO 13485