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Column Care in HILIC
pH Stability
Thermo Scientific HILIC columns are silica based. A suitable pH range is
2−8.
Washing Conditions
Prior to storage, buffered solutions used during HILIC analysis should be
washed out of the column (and the HPLC system). A suitable wash
solution is 60% organic solvent in water.
Storing Conditions
HILIC columns should be stored under HILIC conditions. A mixture of
organic solvent and water in a 90:10 ratio is recommended for storage.
The Product Manual will give specific advice.
Ensure the column end-fittings are sealed with end-plugs to prevent the
stationary phase from drying.
As with any other column, to maximize HILIC column life ensure that samples and mobile
phases are particle-free. We also recommend the use of guard columns or inline filters
between the switching valve and the column.
