38
80 Å
Hydrophobicity
pH Range
Pore Size
2.6 µm
14
High
2 to 8
Low
0
0
300
Particle Size
The urea phase bonded onto solid core particles provides a unique selectivity for polar analytes.
Accucore Urea-HILIC provides good retention for charged and neutral polar compounds.
Accucore Urea-HILIC
(Tb/Tp)
(2dG/3dG)
k U /2
(CX) /2
(OH)
(V/A)
(AX)
(CH2)
This neutral material offers good hydrophilic selectivity and
low ion exchange activity.
NH
2
N
H
O
0.2
0.4
0.6
0.8
1.0
1.2
1.4
0
200
400
600
1
2
3
Time (min)
mAU
0
Column:
Accucore Urea-HILIC
, 2.6 µm
100 × 2.1 mm
Part Number:
27726-102130
Mobile Phase:
composition 10:80:10, A : B : C
A: water
B: acetonitrile
C: 100 mM ammonium acetate adjusted to pH 4.9
Flow Rate:
300 µL/min
Inj.Volume:
2 µL into 10 µL partial loop mode
Injection Wash
Solvent:
water:acetonitrile 20:80
Temp.:
35 °C
Detection:
UV at 230 nm
Backpressure:
71 bar
Run Time :
2 minutes
Acetaminophen
Salicylic acid
Aspirin
t
R
A
s
t
R
A
s
R
s
t
R
A
s
R
s
Mean 0.760 1.474 0.908 1.303 2.359 1.100 1.318 3.264
CV % 0.00 1.17 0.48 0.92 0.49 0.00 0.63 0.48
Data from eight replicate analyses of a mixture of acetaminophen, salicylic acid
and aspirin
Retention time (t
R
), peak asymmetry (A
s
), peak resolution (R
s
)
Analgesic compounds
