35
Syncronis HILIC provides enhanced retention of charged and neutral polar compounds. Outstanding peak shape and
sensitivity are demonstrated on this phase.
Syncronis HILIC stationary phase carries a zwitterionic functional group of the sulfobetaine type, monomerically bonded to
highly pure, high surface area silica. The sulfobetaine zwitterion has both positive (quaternary ammonium) and negative
(sulfonic acid) groups in a 1:1 ratio, so that the net surface charge is zero. Due to counterbalanced electrostatic forces,
which lead to weaker secondary electrostatic interactions with the analyte, lower buffer concentrations are required to
disrupt these interactions. The charge density of this phase is pH-independent and the optimization of the mobile phase
pH is solely dictated by the analyte.
Syncronis HILIC
(Tb/Tp)
(2dG/3dG)
k U /2
(CX) /2
(OH)
(V/A)
(AX)
(CH2)
N
H
3
C
CH
3
SO
3
Relative Abundance
Time (min)
0.5
0.0
10
0
20
30
40
50
60
70
80
90
100
1.0
1.5
2.0
2.5
0.5
0.0
10
0
20
30
40
50
60
70
80
90
100
1.0
1.5
2.0
2.5
0.5
0.0
10
20
30
40
50
60
70
80
90
100
1.0
1.5
2.0
2.5
0.5
0.0
20
30
40
50
60
70
80
90
100
1.0
1.5
2.0
2.5
Benzyldimethylphenylammonium chloride
Neostigmine
Edrophonium
Pyridostigmine
The zwitterionic material offers the highest absolute retention
and good hydrophobic, hydrophilic and isomeric selectivity.
Low ion exchange properties are demonstrated by this neutral
material.
Analysis of edrophonium, neostigmine and pyridostigmine
from plasma by LC-MS/MS
Column:
Syncronis HILIC
, 1.7 µm,
100 mm × 2.1 mm
Part Number:
97502-102130
Mobile Phase:
acetonitrile / ammonium formate,
100 mM, pH 3.3 (90:10 v/v)
Gradient:
5–55% B in 60 min
Flow Rate:
500 µL/min
Inj.Volume:
10 µL
Temp.:
25 °C
Detection:
MS
2 to 8
5
100
1.7 µm, 3 µm, 5 µm
