37
Acclaim HILIC-10 is based on high purity, fully porous silica covalently modified with a proprietary hydrophilic layer.
The cation exchange properties identified on this material are due to the presence of residual deprotonated silanol groups
(under the mobile phase pH conditions used for the characterisation testing).
Acclaim HILIC-10 provides good retention for charged and neutral polar compounds.
This phase shows dual mode retention behavior, with a ‘’U-shaped’’ retention profile as a function of acetonitrile content
in the mobile phase. Increasing retention is demonstrated at both low and high levels of acetonitrile (RPLC and HILIC
modes, respectively).
Acclaim HILIC-10
(Tb/Tp)
(2dG/3dG)
k U /2
(CX) /2
(OH)
(V/A)
(AX)
(CH2)
This material offers good hydrophilic selectivity. Some amount
of cation exchange properties are demonstrated by this slightly
acidic phase.
2 to 8
120
3 µm, 5 µm
Column:
Acclaim HILIC-10
, 3 μm
150 mm × 4.6 mm
Part Number:
074257
Mobile Phase:
90/10 v/v acetonitrile/10 mM (total)
ammonium acetate, pH 5
Flow Rate:
1 mL/min
Inj.Volume:
2 µL
Temp.:
30 °C
Detection:
UV at 230 nm
Sample:
1.Acetaminophen
0.1 mg/mL
2. Salicylic acid
0.1
3.Aspirin
0.2
4. Penicillin G
0.1
5. Metformin
0.1
2
250
mAU
3
0
6
9
12
1
2
3
4
5
Time (min)
Pharmaceuticals
Functionality: Proprietary Hydrophilic Layer
