36
150 Å
Hydrophobicity
pH Range
Pore Size
2.6 µm
14
High
2 to 8
Low
0
0
300
Particle Size
• Amide phase bonded onto 150 Å pore diameter solid core particles
• High retention of a broad range of hydrophilic analytes in HILIC mode
• Recommended for hydrophilic biomolecules such as glycans
The amide bonded phases provide strong hydrogen bonding interaction between the stationary phase and the analytes,
resulting in unique selectivity compared to other HILIC phases. Combined with larger pore size of the solid core particles,
Accucore 150-Amide-HILIC is well suited for separating a variety of hydrophilic molecules, including carbohydrates and
peptides. As a result the Accucore 150-Amide-HILIC is an excellent choice for glycan separations.
A slight amount of cation exchange properties are demonstrated by this slightly acidic phase, possibly due to the
presence of residual deprotonated silanol groups (under the mobile phase pH conditions used for the characterization
testing).
Accucore 150-Amide-HILIC
(Tb/Tp)
(2dG/3dG)
k U /2
(CX) /2
(OH)
(V/A)
(AX)
(CH2)
This material offers good hydrophobic, hydrophilic and
isomeric selectivity and some cation exchange properties.
Column:
Accucore 150-Amide-HILIC
, 2.6 μm,
100 × 2.1 mm
Part Number:
16726-102130
Mobile Phase A:
Acetonitrile
Mobile Phase B:
50 mM ammonium formate (pH 4.5)
Gradient:
Time (min) B%
0
20
40
50
45
50
45.5
20
50
20
Flow Rate:
500 µL/min
Inj.Volume:
2 µL
5 µL
Temp.:
60 °C
Detection:
Fluorescence excitation wavelength: 330 nm
Fluorescence emission wavelength: 420 nm
Sample:
2-aminobenzamide labelled dextranladder
Backpressure:
110 bar
n
CH
2
HC
C=O
NH
2
30
35
40
45
500000
600000
700000
800000
900000
1000000
1100000
1200000
5
10
15
20
25
30
35
40
45
-200000
-100000
0
100000
200000
300000
400000
Time (min)
1
Counts
2
3
4
5
6
7
8 9 10 11
10
11
15
12
14
13
16 17 18 19 20 21
Minutes
300000
200000
0
100000
50000
350000
250000
150000
A
B
2-AB labelled dextran ladder
(A) 2 μL injection of sample, where 11 glycans were separated.
(B) 5 μL injection of sample, zoomed-in to the later part of the
gradient rise. A further 10 glycans were detected.
