40
Hydrophobicity
pH Range
Pore Size
Particle Size
2.6 µm
0
14
2 to 8
Low
High
0
300
80Å
The cation exchange properties identified on this material are due to the presence of residual deprotonated silanol groups
(under the mobile phase pH conditions used for the characterization testing).
Accucore HILIC is recommended for the analysis of basic or neutral polar compounds.
Accucore HILIC
(Tb/Tp)
(2dG/3dG)
k U /2
(CX) /2
(OH)
(V/A)
(AX)
(CH2)
This bare silica material offers good hydrophobic and
hydrophilic selectivity. Good absolute retention and some
isomeric selectivity are demonstrated. Very strong cation
exchange properties exhibited by this acidic phase.
mAU
Time (min)
0
0.5
1
1.5
2.5
2
3
3.5
4
1
2
4
5
6
7
3
0
20
40
60
80
100
120
140
Column:
Accucore HLIC
2.6 µm,
50 × 2.1 mm
Part Number:
17526-052130
Mobile Phase:
85:15 (v/v) acetonitrile/100mM
ammonium formate, pH 3.2
Flow Rate:
2 mL/min
Inj.Volume:
5 µL
Temp.:
40 °C
Detection:
UV at 280 nm
Backpressure:
157 bar
Analytes:
1. Catechol
2. 5-HIAA
3. DOPAC
4. Serotonin
5. L-tyrosine
6. Dopamine
7. L-DOPA
Functionality: Unbonded Silica
Catecholamines
