Thermo Scientific
| Reagents, Solvents and Accessories
21
MethElute Reagent (TMPAH)
A powerful reagent for accurate, sensitive on-column
methylation.
Thermo Scientific MethElute Reagent [0.2 M
trimethylanilinium hydroxide (TMPAH) in methanol solution]
is a powerful methylating reagent for quantitative
methylation and detection of barbiturates, sedatives,
xanthine bases, phenolic alkaloids and Dilantin by gas
chromatography.
Our MethElute Reagent gives a single quantitative peak
response for each substance. When the reagent is heated
with drug-containing extracts, serum or urine, those drugs
containing reactive amino, hydroxyl and carboxyl functions
will be methylated at the reactive sites.
Performance Characteristics
Accuracy.
Comparable or better than the UV/TLC method.
When phenobarbital and Dilantin are present, the UV/TLC
method cannot be used, as Dilantin interferes with the
phenobarbital determination. The GC procedure yields
excellent results for this combination of drugs.
Precision.
The coefficient of variation is 5% or less.
Sensitivity.
Detects barbiturates down to 0.2 mg/dl.
Ordering Information
Product # Description
Pkg. Size
TS-49300
MethEIute Reagent (TMPAH)
10 ml Hypo-Vial
Sample Storage Vial
TS-49301
MethElute Reagent (TMPAH)
12 x 1 ml Hypo-Vial
Sample Storage Vials
[CH
3
–N–CH
3
]
[OH]
CH
3
TMPAH
heat
Methylcarbonium
Ion
Methylated
Phenobarbital
[CH
3
]
O
N
N
CH
3
CH
3
O
O
C
2
H
5
C
5
H
5
O
HN
HN
O
O
C
2
H
5
C
6
H
5
Figure 1.
Thermo Scientific MethElute Reagent reaction with phenobarbital.
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