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For more information, or to download product instructions,
Tri-Sil TP Reagent (TMSI:pyridine)
Great for derivatizing hydroxyl compounds.
Thermo Scientific Tri-Sil TP Reagent is a mixture of TMSI in
dry pyridine (1.5 mEq/ml). It is used primarily for derivatizing
hydroxyl compounds, particularly carbohydrates. Tri-Sil TP
Reagent has been used successfully for the silylation of
alcohols and phenols, organic acids, hydroxylamines, amino
acids, carbohydrates, flavonoids, glycols and polyglycols,
nucleotides, steroids, hydroxyl acids, barbiturates,
narcotics, indoles, and vitamins. Tri-Sil TP Reagent does
not react with amines.
Our Tri-Sil TP Reagent can be used in the presence of water
as long as there is enough reagent present to react with
both the water and the sample. The reagent reads with
water in a 2:1 ratio.
PROTOCOL 1
1. Combine 10-15 mg sample and 1.0 ml Tri-Sil TP Reagent in a 3.0 ml Thermo
Scientific Reacti-Vial Small Reaction Vial.
2. Cap vial and shake to dissolve. If necessary, heat at 60-70°C. Silylation is
complete upon dissolution.
3. Analyze by gas chromatography.
PROTOCOL 2
For solutions containing ~1% or less total sugars, use a 50:50 v/v
TMSI/pyridine solution.
1. Evaporate ~50 µl sugar solution to a glassy syrup in a 0.3 ml Thermo
Scientific Reacti-Vial Small Reaction Vial.
2. Add 50 µl Tri-Sil TP Reagent.
3. Cap vial and heat at 60°C to dissolve and derivatize the sugars.
4. Analyze directly by gas chromatography.
Ordering Information
Product # Description
Pkg. Size
TS-49230
TrI-Sil TP Reagent
10 x 1 ml
TMSI: Pyridine (1:4)
ampules
TS-49231
TrI-SiI TP Reagent
25 ml
TMSI: Pyridine (1:4)
Hypo-Vial Sample
Storage Vial
Silylation Grade Solvents
Manufactured to meet your exacting silylation needs.
Thermo Scientific Silylation Grade Solvents are specially
manufactured and packaged to meet the exacting needs of
silylation and other sensitive derivatization reactions.
Each Silylation Grade Solvent is purified, dried and
packaged under nitrogen in our convenient Hypo-ViaI
Sample Storage Vials. Supplied complete with elastomer
septa, this unique packaging allows immediate access to
your sample, without exposure to moisture and oxygen.
Highlights:
• Purified, dried and packaged under nitrogen in convenient
Hypo-Vial Sample Storage Vials
• Supplied with elastomer septa, allowing immediate access
to sample without exposure to moisture and oxygen
• Use polar solvents (acetonitrile, dimethylformamide,
dimethylsulfoxide, pyridine and tetrahydrofuran) to
facilitate reactions; nonpolar organic solvent may be
used, but they will not accelerate the rate of reaction
• Avoid water or alcohol because TMS reagents react with
active hydrogen; avoid enolizable ketones
• Use dimethylformamide for steroids and other large
molecules
• Use dimethylsulfoxide to prepare TMS derivatives of tertiary
alcohols and some compounds with reluctant solubility in
other silylation solvents
• Pyridine is an excellent solvent and reaction medium for
MS reactions and is an HCI acceptor in reactions involving
organochlorosilanes
• Other commonly used solvents include tetrahydrofuran
and acetonitrile
Ordering Information
Product # Description
Pkg. Size
✖
TS-20062
Acetonitrile
50 ml Hypo-Vial
Sample Storage Vial
✖
TS-20672
Dimethylformamide
50 ml Hypo-Vial
Sample Storage Vial
✖
TS-20684
Dimethylsulfoxide
50 ml Hypo-Vial
Sample Storage Vial
✖
TS-27530
Pyridine
50 ml Hypo-Vial
Sample Storage Vial
✖
TS-27860
Tetrahydrofuran
50 ml Hypo-Vial
Sample Storage Vial
✖
Additional hazardous handling charge.
For HPLC Grade Solvents, see page 42.
Thermo Scientific Silylation Reagents
C
H
H
H
C
Acetonitrile
MW 41.05
bp 81.6
˚
C
N
Dimethylformamide
MW 73.09
bp 153
˚
C
H
CH
3
N
C
CH
3
O
Dimethylsulfoxide
MW 78.13
bp 189
˚
C
CH
3
CH
3
O
S
N
Pyridine
MW 79.10
bp 115.2
˚
C
O
Tetrahydrofuran
MW 72.10
bp 66
˚
C
