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15
Thermo Scientific
Acylation Reagents
Acylation and Acylation Reagents
Acylation is the conversion of compounds (through the
action of a carboxylic acid or a carboxylic acid derivative)
that contain active hydrogens such as -OH, -SH and -NH
esters; thioesters; and amides.
1
In chromatographic
applications, the acylation reaction is used primarily for
converting the above classes of compounds into derivatives
that are better suited for chromatography
2
or that give a
greater response to the chromatographic detection system
than the parent compound.
3
An important example of this application is the insertion of
perfluoroacyl groups into a molecule to enhance the
detectability of the substance by electron capture. The
presence of a carbonyl group adjacent to the halogenated
carbons enhances the electron capture detector (ECD)
response.
Acyl derivatives also are useful in MS applications in which
they direct the fragmentation patterns of the compounds to
be studied.
4
References
1. Donike, M. (1973). Acylation with bis
(acylamides).
N-
Methyl-bis
(trifluoroacetamide), two new reagents for trifluoroacetylation.
J. Chromatogr.
78
, 273-279.
2. Sullivan, J.E. and Schewe, L.R. (1977). Preparation and gas chromatography
of highly volatile trifluoroacetylated carbohydrates using
N
-Methyl-bis
(trifluoroacetamide).
J. Chromatogr. Sci.
15
, 196-197.
3. Benington, F.,
et al.
(1975). Identification and separation of indolealkylamines
by gas liquid chromatographic analysis of their heptafluorobutyryl derivatives.
J. Chromatogr.
106
, 435-439.
4. Borga, 0.,
et al.
(1971). Quantitative determination of nortriptyline and
desmethylnortriptyline in human plasma by combined gas
chromatography-mass spectrometry.
J. Chromatogr.
4(12)
, 837-849.
Pentafluoropropanol
Purified for GC/MS use.
F–C–C–C–OH
F F H
F F H
20
Pentafluoropropanol
MW 150.05
bp 80.6
˚
C
d
4
1.2880
Highlights:
• Addition of fluorine atoms into compounds greatly
enhances the sensitivity of certain detectors for those
materials
• It is advantageous to introduce fluorine atoms for ECD and
GC/MS applications
• Carboxylic acids can be derivatized using a two-step
reaction involving reaction with an anhydride, followed by
a fluorinated alcohol
Ordering Information
Product # Description
Pkg. Size
TS-65195
Pentafluoropropanol
10 x 1 ml
ampules
Servo+ and Servo
•
Ready to use solutions
•
High reproducibility
•
High extract cleanliness
•
Confidence in results
SPE Solutions
for drugs of abuse
