Thermo Scientific
| Reagents, Solvents and Accessories
13
Tri-Sil BP Reagent (BSA:pyridine)
A reagent-solvent solution for one-step derivatization.
Thermo Scientific Tri-Sil BP Reagent is composed of BSA
(2.5 mEq/ml*) and Pyridine.
*1.25 mEq for amides
Tri-Sil BP Reagent reacts with:
• Alcohols, phenols, some enols and other hydroxyl and
polyhydroxyl compounds to form trimethylsilyl ethers
• Organic acids to form trimethylsilyl esters
• Aromatic amides to form N-trimethylsilyl derivatives
• Amines to form N-trimethylsilyl derivatives
In addition, Tri-Sil BP Reagent is excellent for unhindered
steroids, but it is not recommended for carbohydrates.
PROTOCOL
1. Combine 5-10 mg sample and 1.0 ml Tri-Sil BP Reagent in a 3.0 ml Thermo
Scientific Reacti-Vial Small Reaction Vial.
2. Cap vial and heat at 60-70°C for 15-20 minutes to facilitate dissolution and
derivatization.
3. Analyze by gas chromatography.
Ordering Information
Product # Description
Pkg. Size
TS-49012
Tri-SiI BP Reagent
25 ml
(2.5 mEq/ml BSA in pyridine)
Hypo-Vial Sample
Storage Vial
Tri-Sil TBT Reagent (TMSI:BSA:TMCS)
A powerful catalyzed silylation reagent formulation.
Thermo Scientific Tri-Sil TBT is a mixture containing three
parts TMSI, three parts BSA and two parts TMCS. Our
Tri-Sil TBT Reagent converts all classes of hydroxyl groups
to TMS ethers. Under usual conditions, the reaction is
complete in a short period of time at 60-80°C. Highly
hindered hydroxyls may require several hours.
PROTOCOL
This method is used to silylate all hydroxyl groups in steroid structures,
even the most sterically hindered, such as the 17 hydroxyl groups in
cortol. This method also has been used by Bacon and Kokenakes to
measure plasma prednisolone by GC.
1. Combine 0.1-5.0 mg sample and 0.2-0.4 ml Tri-Sil TBT Reagent in a 1.0 ml
Thermo Scientific Reacti-Vial Small Reaction Vial.
2. Cap vial and shake to dissolve.
3. Heat at 60-80°C for 6-24 hours to complete reaction.
4. Analyze by gas chromatography.
NOTE:
If sample is insoluble, add 0.1-0.2 ml pyridine.
Ordering Information
Product # Description
Pkg. Size
TS-49016
Tri-SiI TBT Reagent
10 x 1 ml
TMSI: BSA: TMCS (3:3:2)
ampule
