8
For more information, or to download product instructions,
Thermo Scientific Silylation Reagents
BSTFA
For excellent chromatographic separations.
20
BSTFA
MW 257.4
bp 40
˚
C/12 mm
d
4
0.961
C
CF
3
N
Si
CH
3
O
Si
CH
3
CH
3
CH
3
CH
3
CH
3
The greatest advantage of using Thermo Scientific BSTFA
over other silylating reagents is the increased volatility
of its byproducts, mono(trimethylsilyl) trifluoroacetamide
and trifluoroacetamide. This increased volatility results
in the byproducts eluting with the solvent front, providing
excellent chromatographic separations.
BSTFA is a powerful trimethylsilyl donor, with donor strength
that is comparable to its unfluorinated analog BSA
[
N,O
-Bis(trimethylsilyl)acetamide]. BSTFA reacts to replace
labile hydrogens on a wide range of polar compounds with
a -Si(CH
3
)
3
group. This physical characteristic is particularly
useful in the gas chromatography of some lower boiling
TMS-amino acids and TMS Krebs cycle acids.
PROTOCOL
1. Combine 5-10 mg sample, 0.5 ml BSTFA and 1.0 ml solvent (acetonitrile is
recommended for amino acids) in a 3.0 ml Thermo Scientific Reacti-ViaI
™
Small Reaction Vial.
2. Cap vial and shake for 30 seconds.
3. Heat at 70°C for 15 minutes.
4. Analyze by gas chromatography.
NOTE:
This protocol is not recommended for sugars.
Ordering Information
Product # Description
Pkg. Size
TS-38828
BSTFA
25 g
[
N,O-
bis (trimethylsilyl)
Hypo-Vial Sample
trifluoroacetamide]
Storage Vial
✖
TS-38829
BSTFA
100 g
Hypo-Vial
™
Sample
Storage Vial
TS-38830
BSTFA
10 x 1 ml
ampules
✖
Additional hazardous handling charge.
BSTFA + TMCS
The reagent to choose for difficult-to-silylate compounds.
20
BSTFA
MW 257.4
bp 40
˚
C/12 mm
d
4
0.961
C
CF
3
N
Si
CH
3
O
Si
CH
3
CH
3
CH
3
CH
3
CH
3
20
TMCS
MW 108.7
bp 57.6
˚
C
d
4
0.858
Si
CH
3
CH
3
CH
3
CI
Thermo Scientific BSTFA + 1% TMCS is ideal for
derivatizing fatty acid amides, slightly hindered hydroxyls
and other difficult-to-silylate compounds. This catalyzed
formulation is stronger than BSTFA alone.
PROTOCOL
1. Combine 5-10 mg sample, 0.5 ml BSTFA + 1% TMCS and 1.0 ml solvent
(acetonitrile is recommended for amino acids) in a 3.0 ml Thermo
Scientific Reacti-Vial Small Reaction Vial.
2. Cap vial and shake for 30 seconds.
3. Heat at 7°C for 15 minutes.
4. Analyze by gas chromatography.
Ordering Information
Product # Description
Pkg. Size
TS-38831
BSTFA + 1% TMCS
10 x 1 ml
[
N-O-
bis
(trimethylsilyl)
ampules
trifluoroacetamide +
1% Trimethylchlorosilane]
TS-38832
BSTFA + 1% TMCS
10 g
Hypo-Vial Sample
Storage Vial
TS-38833
BSTFA + 1% TMCS
25 g
Hypo-Vial Sample
Storage Vial
✖
TS-38834
BSTFA + 1% TMCS
100 g
Hypo-Vial Sample
Storage Vial
TS-38840
BSTFA + 10% TMCS
10 x 1 ml
[
N-O-
bis
(trimethylsilyl)
ampules
trifluoroacetamide +
10% Trimethylchlorosilane]
✖
Additional hazardous handling charge.
