2
For more information, or to download product instructions,
Derivatization Reagents for Specific Functional Groups (Continued)
Functional Group
Procedure
Reagent
Derivative
Notes
Silylation
TMSI
TMS
Acylation
HFAA
Heptafluorobutrates
PFAA
Pentafluoropropionates
TFAA
Trifluoroacetates
HFBI
Heptafluorobutrates
Silylation
MSTFA
TMS
TMSI
TMS
Can be used with some syrups
Tri-Sil Reagents
TMS
Acylation
MBTFA
Trifluoroacetates
Volatile derivatives of mono-, di-
TFAI
Trifluoroacetates
and trisaccharides
Silylation
BSA
TMS
Easily formed, generally not stable,
analyze quickly
BSTFA
TMS
BSTFA+TMCS
TMS
MSTFA
TMS
TMCS
TMS
Can be used with some salts
TMSI
TMS
Tri-Sil Reagents
TMS
MTBSTFA
TBDMS
More stable than TMS derivatives
MTBSTFA+TBDMCS
TBDMS
TBDMCS aids derivatization
Alkylation
PFBBr
Pentafluorobenzyl Esters
Used in EC detection & UV, MS
BF
3
-Methanol
Methyl Esters
Best for large samples of fatty acids
Methylate Reagent (DMFDMA) Methyl Esters
Fatty acids and amino acids
MethElute Reagent (TMPAH)
Methyl Esters
On-column derivatization
PFAA+Pentafluoropropanol
Pentafluoropropyl Ester
Drug analysis
Silylation
BSTFA
TMS
MTBSTFA
TBDMS
Acylation
HFAA
Heptafluorobutrates
Silylation
BSTFA
TMS
Acylation
HFAA
Heptafluorobutrates
PFAA
Pentafluoropropionates
TFAA
Trifluoroacetates
Carboxyl
O
–C–OH
Carbohydrates
(CH
2
OH)
n
Catecholamines
Inorganic Anions
Sulfonamides
Nitrosamines
OH
OH
N
R
O
R
N
N
N
R
O O
R
R
